A 12-step user guide for analyzing voxel-wise gray matter asymmetries in statistical parametric mapping (SPM).

Kurth F; Gaser C; Luders E;
Nature protocols. 2015-Feb; 10(293-304):2
Voxel-based morphometry (VBM) has been proven capable of capturing cerebral gray matter asymmetries with a high (voxel-wise) regional specificity. However, a standardized reference on how to conduct voxel-wise asymmetry analyses is missing. This protocol provides the scientific community with a carefully developed guide describing, in 12 distinct steps, how to take structural images from data pre-processing, via statistical analysis, to the final interpretation of the significance maps. Key adaptations compared with the standard VBM workflow involve establishing a voxel-wise hemispheric correspondence, capturing the direction and degree of asymmetry and preventing a blurring of information across hemispheres. The workflow incorporates the most recent methodological developments, including high-dimensional spatial normalization and partial volume estimations. Although the protocol is primarily designed to enable relatively inexperienced users to conduct a voxel-based asymmetry analysis on their own, it may also be useful to experienced users who wish to efficiently adapt their existing scripts or pipelines.
PMID: 25591011    doi: 10.1038/nprot.2015.014

BMAP Authors

Eileen Luders
Eileen Luders Ph.D.
Florian Kurth
Florian Kurth M.D. Ph.D.